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Simple method for rapid quantification of branched-chain 2-oxo acids in physiological fluids as quinoxalinol derivatives
Summary
Type:
HPLC
Application #:
100140
Catalog#:
720041.40 - MN HPLC Column, Nucleosil C18, 120A, 5um, 4x250mm
Detection:
fluorescence, excitation 350nm, emission 410nm
Conditions:
eluent: methanol - water (55:45, v/v),
flow rate 1.1ml/min
Authors:
Radeck W
Source:
J Chromatogr 432 (1988) 297 - 301
Details
Legend: Fig. 1: Chromatogram of quinoxalinols of 2-oxo acids in equimolar concentrations, 1. oxaloacetate, 2-oxoglutarate and pyruvate, 2. 2-oxobutanoate, 3. 3-methyl-2-oxobutanoate (KIV), 4. 4-methyl-
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